IBBIOTECH

Experiencia y futuro en biotecnología

Curettage researches

The research of curettage by means of FISH, consists of marking the chromosomes under study with DNA catheters linked to fluorochromes. The analysis of the marking result through fluorescence microscopy lets us identify the number of copies for a certain chromosome and possible structural alterations. Using this technique in trophoblastic cells of an uncultured obstetric curettage, most frequent chromosome abnormalities can be detected, such us Down Syndrome, Edward Syndrome, Turner Syndrome, Patau Syndrome, etc. The main advantage of FISH for this kind of samples is the difficulty to obtain a complete kariotype, since in many occasions the cell growth is not detected and by means of FISH we detect the most frequent abnormalities responsible for miscarriages. In this case we include the research of the chromosomes 22 and 16.

In a complementary way, we carry out the determination of numerical or structural abnormalities in the complete chromosome dotation by means of the culture of the trophoblastic cells of an obstetric curettage and the cytogenetic techniques. Therefore, a metaphase analysis is carried out from the cells in culture through the CTG banding technique.

The importance of the curettage research is that it enables us to know the possible genetic base in the pregnancy loss and to control the risk of possible foetal losses.

In IB Biotech, within 24/48 hours we inform you about the result of the FISH, which will be complemented with the genetic kariotype in the following 15 days.

How to send your sample?